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Purification of recombinant proteins The plasmids, containing a codon-optimized human SERF2 gene, were synthesized commercially by GenScript and subcloned to a pER28a-SUMO vector as reported elsewhere 39,42

2.3 Mitochondrial drive for lipid peroxidation Ferroptosis's defining hallmark is iron-dependent lipid peroxidation at the plasma membrane, driven by GPX4 inactivation and glutathione depletion, with mitochondrial changes often observed as secondary features

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On the next day my resting shoulder was aching and I couldnt raise my arm without pain